On the relationship between bluetongue, African horsesickness and reoviruses: hybridization studies.
نویسندگان
چکیده
VERWOERD, D. W. & HUISMANS, H . On the relationship between bluetongue, Afr ican horsesickness and reo viruses : hybridization studies. Onderstepoort J. vet. Res., 36 (2) , 175-180, 1969. The double-stranded ribonucleic acid from bluetongue virus (BTV) , African horsesickness virus (AHSV) and reovirus has been tested for hybridization with messe~ger RNA derived from BTV and reovirus-infected cells. No relationship was found between reov1rus and BTV or AHSV, but a small amount of hybridization between BTV and AHSV did occur.
منابع مشابه
Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes.
The serogroup specificity of the bluetongue virus (BTV) NS1 and VP3 gene probes was confirmed by means of northern blot hybridization. Under high-stringency conditions both probes hybridized to 22 BTV serotypes (18 South African serotypes, BTV3 from Cyprus and BTV16 from Pakistan) but not to serotypes that originate from Australia and India. Furthermore, NS1 gene probes of BTV and African horse...
متن کاملPlaque formation by African horsesickness virus and characterization of its RNA.
OELLERMANN, R. A. _Plaque formation by African horsesickness virus and characterization of its RNA. Ondmtepoort j. vet. Ru. (1970), 37 (2), 137144. A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus producti...
متن کاملThe use of a membrane feeding technique to determine the infection rate of Culicoides imicola (Diptera, Ceratopogonidae) for 2 bluetongue virus serotypes in South Africa.
Culicoides spp. in the Lowveld of the northern Transvaal, Republic of South Africa, were fed bluetongue virus serotypes 3 and 6 and African horsesickness virus serotype 1 through latex and chicken skin membranes. After an incubation period of 10 days at 25-27 degrees C, the infection rate of C. imicola for bluetongue virus serotypes 3 and 6 was established at 31% and 24% respectively. No Africa...
متن کاملThe complete sequence of African horsesickness virus serotype 4 (vaccine strain) RNA segment 5 and its predicted polypeptide compared with NS1 of bluetongue virus.
The complete sequence of RNA segment 5 of the African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from cDNA clones inserted into pBR322. The RNA is 1751 bp long (M(r) 1.12 x 10(6)) and contains an open reading frame encoding a protein of 548 amino acids (M(r) 63,122) with a net charge of +0.5 at neutral pH. A comparison of the sequence of AHSV-4 segment 5 with that of ...
متن کاملCharacterization and cloning of the African horsesickness virus genome.
The dsRNA profiles of all nine African horsesickness virus (AHSV) serotypes were compared by agarose gel electrophoresis and PAGE. The agarose profiles were identical, but a unique profile was obtained for each of the nine serotypes by PAGE. Nine of the 10 dsRNA genome segments of AHSV-3 were cloned and the clones were used in dot-spot and Northern blot hybridization experiments to determine in...
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 36 2 شماره
صفحات -
تاریخ انتشار 1969